frozen mouse adipose tissues  (Miltenyi Biotec)

Journal: Scientific Reports

Article Title: Olfactory receptors are expressed in pancreatic β-cells and promote glucose-stimulated insulin secretion

doi: 10.1038/s41598-018-19765-5

Figure Lengend Snippet: OA-induced enhancement of GSIS from MIN6 cells is abrogated by knockdown of OLFR15. ( a ) Protein expression levels of OLFR15 and ACTIN in MIN6 cells treated with NT siRNA or Olfr15 siRNA (L-064657-01) for 72 hours. Full-length blots are presented in Supplementary Fig. . ( b ) Densitometric analyses for NT siRNA (n = 5) and Olfr15 siRNA (L-064657-01) (n = 6) shown in ( a ). ( c ) MIN6 cells were transfected with NT siRNA or Olfr15 siRNA (L-064657-01) for 72 hours, followed by monitoring for insulin secretion at 16.7 mM glucose with or without OA (n = 5 or 6 per group). Eight-week-old C57BL/6 J male mice were subjected to glucose tolerance tests performed in 30 minutes after an oral administration of dimethyl sulfoxide (DMSO) (n = 4) or OA (n = 5), followed by measurement of ( d ) blood glucose levels during the tests and ( e ) plasma insulin levels 15 minutes after glucose loading. NT, non-targeting. unpaired Student’s t test ( b and e ), one-way ANOVA ( c ) and two-way ANOVA ( d ): * P < 0.05. NS, not significant. Data are presented as means ± SE.

Article Snippet: Proteins derived from brain, heart, lung, liver, pancreas, kidney, small intestine, colon, testis and adipose tissue in C57BL/6 mice were purchased from Zyagen Laboratories (San Diego, CA, USA).

Techniques: Expressing, Transfection